The GLM Procedure Class Level Information Class Levels Values sample 6 1 2 3 4 5 6 tissue 2 flo veg Number of observations 12 The GLM Procedure Dependent Variable: conc1 Sum of Source DF Squares Mean Square F Value Pr > F Model 6 28.01059667 4.66843278 3.01 0.1233 Error 5 7.74841300 1.54968260 Corrected Total 11 35.75900967 R-Square Coeff Var Root MSE conc1 Mean 0.783316 15.44271 1.244862 8.061167 Source DF Type III SS Mean Square F Value Pr > F sample 5 2.02684967 0.40536993 0.26 0.9163 tissue 1 25.98374700 25.98374700 16.77 0.0094 The GLM Procedure Dependent Variable: conc2 Sum of Source DF Squares Mean Square F Value Pr > F Model 6 9.45368150 1.57561358 1.15 0.4494 Error 5 6.86250742 1.37250148 Corrected Total 11 16.31618892 R-Square Coeff Var Root MSE conc2 Mean 0.579405 16.15228 1.171538 7.253083 Source DF Type III SS Mean Square F Value Pr > F sample 5 6.38832942 1.27766588 0.93 0.5304 tissue 1 3.06535208 3.06535208 2.23 0.1953 The GLM Procedure Dependent Variable: conc3 Sum of Source DF Squares Mean Square F Value Pr > F Model 6 15.03615900 2.50602650 2.97 0.1263 Error 5 4.21949367 0.84389873 Corrected Total 11 19.25565267 R-Square Coeff Var Root MSE conc3 Mean 0.780870 11.87845 0.918640 7.733667 Source DF Type III SS Mean Square F Value Pr > F sample 5 10.36863767 2.07372753 2.46 0.1731 tissue 1 4.66752133 4.66752133 5.53 0.0654 The GLM Procedure Dependent Variable: conc4 Sum of Source DF Squares Mean Square F Value Pr > F Model 6 13.53428300 2.25571383 17.14 0.0034 Error 5 0.65808867 0.13161773 Corrected Total 11 14.19237167 R-Square Coeff Var Root MSE conc4 Mean 0.953631 4.528574 0.362792 8.011167 Source DF Type III SS Mean Square F Value Pr > F sample 5 6.65163767 1.33032753 10.11 0.0120 tissue 1 6.88264533 6.88264533 52.29 0.0008 The GLM Procedure Dependent Variable: conc5 Sum of Source DF Squares Mean Square F Value Pr > F Model 6 16.30054950 2.71675825 4.59 0.0578 Error 5 2.95880342 0.59176068 Corrected Total 11 19.25935292 R-Square Coeff Var Root MSE conc5 Mean 0.846371 8.872748 0.769260 8.669917 Source DF Type III SS Mean Square F Value Pr > F sample 5 6.98837942 1.39767588 2.36 0.1836 tissue 1 9.31217008 9.31217008 15.74 0.0107 The GLM Procedure Dependent Variable: conc6 Sum of Source DF Squares Mean Square F Value Pr > F Model 6 6.13200400 1.02200067 3.11 0.1166 Error 5 1.64084000 0.32816800 Corrected Total 11 7.77284400 R-Square Coeff Var Root MSE conc6 Mean 0.788901 6.118986 0.572859 9.362000 Source DF Type III SS Mean Square F Value Pr > F sample 5 5.89003600 1.17800720 3.59 0.0935 tissue 1 0.24196800 0.24196800 0.74 0.4298 The GLM Procedure Dependent Variable: conc7 Sum of Source DF Squares Mean Square F Value Pr > F Model 6 20.08224467 3.34704078 2.24 0.1977 Error 5 7.48770500 1.49754100 Corrected Total 11 27.56994967 R-Square Coeff Var Root MSE conc7 Mean 0.728411 11.54019 1.223741 10.60417 Source DF Type III SS Mean Square F Value Pr > F sample 5 17.32320167 3.46464033 2.31 0.1893 tissue 1 2.75904300 2.75904300 1.84 0.2327 The GLM Procedure Repeated Measures Analysis of Variance Repeated Measures Level Information Dependent Variable conc1 conc2 conc3 conc4 conc5 conc6 Level of julian 179 200 221 242 269 290 Repeated Measures Level Information Dependent Variable conc7 Level of julian 320 The GLM Procedure Repeated Measures Analysis of Variance Tests of Hypotheses for Between Subjects Effects Source DF Type III SS Mean Square F Value Pr > F sample 5 6.84272367 1.36854473 1.31 0.3870 tissue 1 35.90198876 35.90198876 34.38 0.0020 Error 5 5.22202152 1.04440430 The GLM Procedure Repeated Measures Analysis of Variance Univariate Tests of Hypotheses for Within Subject Effects Source DF Type III SS Mean Square F Value Pr > F julian 6 93.21110731 15.53518455 17.68 <.0001 julian*sample 30 48.79434783 1.62647826 1.85 0.0484 julian*tissue 6 17.01045807 2.83507635 3.23 0.0144 Error(julian) 30 26.35382964 0.87846099 Adj Pr > F Source G - G H - F julian <.0001 <.0001 julian*sample 0.1316 0.0484 julian*tissue 0.0581 0.0144 Error(julian) Greenhouse-Geisser Epsilon 0.4631 Huynh-Feldt Epsilon 2.3515 The GLM Procedure Least Squares Means tissue conc1 LSMEAN conc2 LSMEAN conc3 LSMEAN conc4 LSMEAN conc5 LSMEAN flo 6.58966667 6.74766667 7.11000000 7.25383333 7.78900000 veg 9.53266667 7.75850000 8.35733333 8.76850000 9.55083333 tissue conc6 LSMEAN conc7 LSMEAN flo 9.50400000 10.1246667 veg 9.22000000 11.0836667 Plot of LSMEAN*julian. Symbol is value of tissue. LSMEAN | | 11.5 + | | | | v 11.0 + | | | | 10.5 + | | | | f 10.0 + | | | | v 9.5 + v f | | | v | 9.0 + | | v | | 8.5 + | v | | | 8.0 + | | v f | | 7.5 + | | f | | f 7.0 + | | | f | f 6.5 + | -+---------+---------+---------+---------+---------+---------+---------+- 180 200 220 240 260 280 300 320 julian The GLM Procedure Class Level Information Class Levels Values tissue 2 flo veg Number of observations 12 The GLM Procedure Repeated Measures Analysis of Variance Repeated Measures Level Information Dependent Variable conc1 conc2 conc3 conc4 conc5 conc6 Level of julian 179 200 221 242 269 290 Repeated Measures Level Information Dependent Variable conc7 Level of julian 320 Partial Correlation Coefficients from the Error SSCP Matrix / Prob > |r| DF = 10 conc1 conc2 conc3 conc4 conc5 conc6 conc7 conc1 1.000000 0.134474 0.041460 -0.043679 0.362419 0.103922 -0.314957 0.6934 0.9037 0.8985 0.2734 0.7611 0.3455 conc2 0.134474 1.000000 0.447446 -0.534294 0.059159 -0.305750 -0.351114 0.6934 0.1676 0.0904 0.8628 0.3605 0.2897 conc3 0.041460 0.447446 1.000000 -0.629701 -0.290414 -0.409596 -0.136772 0.9037 0.1676 0.0379 0.3863 0.2109 0.6884 conc4 -0.043679 -0.534294 -0.629701 1.000000 0.587425 0.365034 0.274047 0.8985 0.0904 0.0379 0.0574 0.2697 0.4148 conc5 0.362419 0.059159 -0.290414 0.587425 1.000000 0.467132 0.003847 0.2734 0.8628 0.3863 0.0574 0.1474 0.9910 conc6 0.103922 -0.305750 -0.409596 0.365034 0.467132 1.000000 0.480414 0.7611 0.3605 0.2109 0.2697 0.1474 0.1347 conc7 -0.314957 -0.351114 -0.136772 0.274047 0.003847 0.480414 1.000000 0.3455 0.2897 0.6884 0.4148 0.9910 0.1347 E = Error SSCP Matrix julian_N represents the nth degree polynomial contrast for julian julian_1 julian_2 julian_3 julian_4 julian_5 julian_6 julian_1 27.39687 6.02920 0.16131 7.86022 -2.10884 -7.69737 julian_2 6.02920 7.77943 2.25399 0.48694 -2.43576 -0.22756 julian_3 0.16131 2.25399 15.51495 -4.92731 -2.80410 5.26278 julian_4 7.86022 0.48694 -4.92731 9.76926 -0.95689 -4.21350 julian_5 -2.10884 -2.43576 -2.80410 -0.95689 6.64213 -2.48450 julian_6 -7.69737 -0.22756 5.26278 -4.21350 -2.48450 8.04554 The GLM Procedure Repeated Measures Analysis of Variance Partial Correlation Coefficients from the Error SSCP Matrix of the Variables Defined by the Specified Transformation / Prob > |r| DF = 10 julian_1 julian_2 julian_3 julian_4 julian_5 julian_6 julian_1 1.000000 0.412986 0.007824 0.480456 -0.156329 -0.518459 0.2068 0.9818 0.1347 0.6462 0.1023 julian_2 0.412986 1.000000 0.205165 0.055856 -0.338850 -0.028763 0.2068 0.5451 0.8704 0.3080 0.9331 julian_3 0.007824 0.205165 1.000000 -0.400225 -0.276226 0.471045 0.9818 0.5451 0.2226 0.4109 0.1436 julian_4 0.480456 0.055856 -0.400225 1.000000 -0.118789 -0.475264 0.1347 0.8704 0.2226 0.7279 0.1396 julian_5 -0.156329 -0.338850 -0.276226 -0.118789 1.000000 -0.339866 0.6462 0.3080 0.4109 0.7279 0.3065 julian_6 -0.518459 -0.028763 0.471045 -0.475264 -0.339866 1.000000 0.1023 0.9331 0.1436 0.1396 0.3065 Sphericity Tests Mauchly's Variables DF Criterion Chi-Square Pr > ChiSq Transformed Variates 20 0.0669579 21.028714 0.3954 Orthogonal Components 20 0.0669579 21.028714 0.3954 Manova Test Criteria and Exact F Statistics for the Hypothesis of no julian Effect H = Type III SSCP Matrix for julian E = Error SSCP Matrix S=1 M=2 N=1.5 Statistic Value F Value Num DF Den DF Pr > F Wilks' Lambda 0.13063553 5.55 6 5 0.0400 Pillai's Trace 0.86936447 5.55 6 5 0.0400 Hotelling-Lawley Trace 6.65488518 5.55 6 5 0.0400 Roy's Greatest Root 6.65488518 5.55 6 5 0.0400 The GLM Procedure Repeated Measures Analysis of Variance Manova Test Criteria and Exact F Statistics for the Hypothesis of no julian*tissue Effect H = Type III SSCP Matrix for julian*tissue E = Error SSCP Matrix S=1 M=2 N=1.5 Statistic Value F Value Num DF Den DF Pr > F Wilks' Lambda 0.21166956 3.10 6 5 0.1173 Pillai's Trace 0.78833044 3.10 6 5 0.1173 Hotelling-Lawley Trace 3.72434493 3.10 6 5 0.1173 Roy's Greatest Root 3.72434493 3.10 6 5 0.1173 The GLM Procedure Repeated Measures Analysis of Variance Tests of Hypotheses for Between Subjects Effects Source DF Type III SS Mean Square F Value Pr > F tissue 1 35.90198876 35.90198876 29.76 0.0003 Error 10 12.06474519 1.20647452 The GLM Procedure Repeated Measures Analysis of Variance Univariate Tests of Hypotheses for Within Subject Effects Source DF Type III SS Mean Square F Value Pr > F julian 6 93.21110731 15.53518455 12.40 <.0001 julian*tissue 6 17.01045807 2.83507635 2.26 0.0491 Error(julian) 60 75.14817748 1.25246962 Adj Pr > F Source G - G H - F julian <.0001 <.0001 julian*tissue 0.0968 0.0577 Error(julian) Greenhouse-Geisser Epsilon 0.5312 Huynh-Feldt Epsilon 0.8868 The GLM Procedure Repeated Measures Analysis of Variance Analysis of Variance of Contrast Variables julian_N represents the nth degree polynomial contrast for julian Contrast Variable: julian_1 Source DF Type III SS Mean Square F Value Pr > F Mean 1 73.71945589 73.71945589 26.91 0.0004 tissue 1 6.44455483 6.44455483 2.35 0.1561 Error 10 27.39687345 2.73968734 Contrast Variable: julian_2 Source DF Type III SS Mean Square F Value Pr > F Mean 1 16.53994026 16.53994026 21.26 0.0010 tissue 1 1.06560438 1.06560438 1.37 0.2690 Error 10 7.77942893 0.77794289 Contrast Variable: julian_3 Source DF Type III SS Mean Square F Value Pr > F Mean 1 1.18002159 1.18002159 0.76 0.4036 tissue 1 0.32201707 0.32201707 0.21 0.6584 Error 10 15.51494771 1.55149477 Contrast Variable: julian_4 Source DF Type III SS Mean Square F Value Pr > F Mean 1 1.04190309 1.04190309 1.07 0.3261 tissue 1 7.62108321 7.62108321 7.80 0.0190 Error 10 9.76925736 0.97692574 Contrast Variable: julian_5 Source DF Type III SS Mean Square F Value Pr > F Mean 1 0.55166841 0.55166841 0.83 0.3836 tissue 1 0.99066582 0.99066582 1.49 0.2500 Error 10 6.64213326 0.66421333 The GLM Procedure Repeated Measures Analysis of Variance Analysis of Variance of Contrast Variables julian_N represents the nth degree polynomial contrast for julian Contrast Variable: julian_6 Source DF Type III SS Mean Square F Value Pr > F Mean 1 0.17811808 0.17811808 0.22 0.6481 tissue 1 0.56653276 0.56653276 0.70 0.4210 Error 10 8.04553677 0.80455368 The GLM Procedure Least Squares Means tissue conc1 LSMEAN conc2 LSMEAN conc3 LSMEAN conc4 LSMEAN conc5 LSMEAN flo 6.58966667 6.74766667 7.11000000 7.25383333 7.78900000 veg 9.53266667 7.75850000 8.35733333 8.76850000 9.55083333 tissue conc6 LSMEAN conc7 LSMEAN flo 9.50400000 10.1246667 veg 9.22000000 11.0836667 The Mixed Procedure Model Information Data Set WORK.CONCS Dependent Variable conc Covariance Structure Variance Components Estimation Method REML Residual Variance Method Profile Fixed Effects SE Method Model-Based Degrees of Freedom Method Containment Class Level Information Class Levels Values tissue 2 flo veg sample 6 1 2 3 4 5 6 wp 12 1 2 3 4 5 6 7 8 9 10 11 12 date 7 179 200 221 242 269 290 320 Dimensions Covariance Parameters 3 Columns in X 24 Columns in Z 18 Subjects 1 Max Obs Per Subject 84 Observations Used 84 Observations Not Used 0 Total Observations 84 Iteration History Iteration Evaluations -2 Res Log Like Criterion 0 1 239.12603637 1 3 239.10235984 0.00001750 2 2 239.10142005 0.00000003 3 1 239.10141850 0.00000000 Convergence criteria met. Covariance Parameter Estimates Cov Parm Estimate sample 0.009434 tissue*sample 0 Residual 1.2365 Fitting Information Res Log Likelihood -119.6 Akaike's Information Criterion -121.6 The Mixed Procedure Fitting Information Schwarz's Bayesian Criterion -121.3 -2 Res Log Likelihood 239.1 Type 3 Tests of Fixed Effects Num Den Effect DF DF F Value Pr > F tissue 1 5 29.04 0.0030 date 6 60 12.56 <.0001 tissue*date 6 60 2.29 0.0465 The Mixed Procedure Model Information Data Set WORK.CONCS Dependent Variable conc Covariance Structures Variance Components, Compound Symmetry Subject Effect tissue*sample Estimation Method REML Residual Variance Method Profile Fixed Effects SE Method Model-Based Degrees of Freedom Method Containment Class Level Information Class Levels Values tissue 2 flo veg sample 6 1 2 3 4 5 6 wp 12 1 2 3 4 5 6 7 8 9 10 11 12 date 7 179 200 221 242 269 290 320 Dimensions Covariance Parameters 3 Columns in X 24 Columns in Z 6 Subjects 1 Max Obs Per Subject 84 Observations Used 84 Observations Not Used 0 Total Observations 84 Iteration History Iteration Evaluations -2 Res Log Like Criterion 0 1 239.12603637 1 1 239.02903841 0.00000000 Convergence criteria met. Covariance Parameter Estimates Cov Parm Subject Estimate sample 0.02315 CS tissue*sample -0.02972 Residual 1.2525 Fitting Information Res Log Likelihood -119.5 Akaike's Information Criterion -122.5 Schwarz's Bayesian Criterion -122.2 The Mixed Procedure Fitting Information -2 Res Log Likelihood 239.0 Type 3 Tests of Fixed Effects Num Den Effect DF DF F Value Pr > F tissue 1 65 34.38 <.0001 date 6 65 12.40 <.0001 tissue*date 6 65 2.26 0.0480 Least Squares Means Standard Effect tissue date Estimate Error DF t Value Pr > |t| tissue*date flo 179 6.5897 0.4557 65 14.46 <.0001 tissue*date flo 200 6.7477 0.4557 65 14.81 <.0001 tissue*date flo 221 7.1100 0.4557 65 15.60 <.0001 tissue*date flo 242 7.2538 0.4557 65 15.92 <.0001 tissue*date flo 269 7.7890 0.4557 65 17.09 <.0001 tissue*date flo 290 9.5040 0.4557 65 20.86 <.0001 tissue*date flo 320 10.1247 0.4557 65 22.22 <.0001 tissue*date veg 179 9.5327 0.4557 65 20.92 <.0001 tissue*date veg 200 7.7585 0.4557 65 17.03 <.0001 tissue*date veg 221 8.3573 0.4557 65 18.34 <.0001 tissue*date veg 242 8.7685 0.4557 65 19.24 <.0001 tissue*date veg 269 9.5508 0.4557 65 20.96 <.0001 tissue*date veg 290 9.2200 0.4557 65 20.23 <.0001 tissue*date veg 320 11.0837 0.4557 65 24.32 <.0001 The Mixed Procedure Model Information Data Set WORK.CONCS Dependent Variable conc Covariance Structures Variance Components, Compound Symmetry Subject Effect tissue*sample Estimation Method REML Residual Variance Method Profile Fixed Effects SE Method Model-Based Degrees of Freedom Method Containment Class Level Information Class Levels Values tissue 2 flo veg sample 6 1 2 3 4 5 6 wp 12 1 2 3 4 5 6 7 8 9 10 11 12 Dimensions Covariance Parameters 3 Columns in X 12 Columns in Z 6 Subjects 1 Max Obs Per Subject 84 Observations Used 84 Observations Not Used 0 Total Observations 84 Iteration History Iteration Evaluations -2 Res Log Like Criterion 0 1 355.44511765 1 1 355.32806426 0.00000000 Convergence criteria met. Covariance Parameter Estimates Cov Parm Subject Estimate sample 0.02315 CS tissue*sample -0.03716 Residual 1.3045 Fitting Information Res Log Likelihood -177.7 Akaike's Information Criterion -180.7 Schwarz's Bayesian Criterion -180.4 -2 Res Log Likelihood 355.3 The Mixed Procedure Type 3 Tests of Fixed Effects Num Den Effect DF DF F Value Pr > F tissue 1 71 0.51 0.4761 date 1 71 1.57 0.2143 date*tissue 1 71 0.37 0.5454 date*date 1 71 1.28 0.2610 date*date*tissue 1 71 0.29 0.5890 date*date*date 1 71 1.05 0.3094 date*date*date*tissu 1 71 0.25 0.6208